Makemson Lab
                        
                   Bacterial Bioluminescence Research

Links to Makemson's Courses:  BCH 3033 General Biochemistry (majors, Fall and Summer)
                                                  PCB6025 Molecular and Cellular Biology 1 (Fall)
                                                  MCB 3020General Microbiology (majors, Spring)             
                                                  MCB 2000 Introductory Microbiology (OnLine, Summer)
                                                  MCB 4653 Food Microbiology (majors, every other Spring)
                                                  SLS 1501 First Year Experience (QBIC only)
                                     BSC 4931  Senior Seminar (majors, Spring)
Link to Makemson's CV

RESEARCH:
Most Recent Student Projects:
    Robin McCollum:  Classification of Bacterial Luciferases-Structure and Kinetics

    Alexis Rodriguez:  Ukraine Luminous Bacteria:  Taxonomy and Luciferase Kinetics

Current Interest:  the Arginine Effect.   And, assay of quorum sensing inhibitors, synthetic and those in Ginseng (Link to Recent Poster Presentations).

    Arginine was the first molecule discovered to induce luciferase synthesis (Coffee, 1968).  Nealson, Platt and Hastings (1970) showed, in a landmark paper, that the arginine effect was only operative during "Autoinduction" of luciferase synthesis.  Today, autoinduction is called "Quorum Sensing" which in Vibrio harveyi is the integration of three autoinducers (produced by the bacteria themselves) through a complex phoshorylation cascade that includes small regulatory RNA's at Lux R the master regulator of the luciferase operon (lux).  We are interested in how arginine, one of the common 20 amino acids, is able to stimulate luciferase synthesis in this system.

   

Luminous Bacteria on Peptone-Yeast Extract-Glycerol Seawater Agar
Both Images the same plate: left by room light, right by the bacterial luminescence.
Can you see the dark mutant colonies?

ARGININE EFFECT on Agar (1 micromole Arg on Disk):


Left:Lights On, 0 time      Middle: Bioluminescence - 0 time    Right: Biolum after  6 hrs

Arginine Effect in HEPES Minimal Broth



The lab group:  students participating in their own research projects.  The lab is equiped with photometers, spectrophotometers, luminescence imaging (chilled CCD), PCR thermalcycler, Agarose Electrophoresis and PAGE, incubators, Luciferase assay photometer, and other biochemical/microbiologcial equipment.

Weekly lab meeting:  discussion of ancient to recent papers on bacterial bioluminescence.