| Course Policies, Required Text, Exam Grade Policies|

Laboratory Link

General Biochemistry

Fall  -  2009
 
Lectures - MWF  2:00-2:50 PM    VH 131
PreTest
  and  Study Guide   Lecture Links (below) are to Handouts.  Example OLD EXAM.
Principles of  STUDY


Instructor Email Phone Office hours
John Makemson
 makemson@fiu.edu
 305-348-3097
 OE 246/7:  MTWTh  11:00 to Noon   and    Friday mornings

Tentative Syllabus

Lecture PowerPoint figures (or Exam Answers and Scores) are available by clicking on the lecture topic for that day. They are all in pdf format.  I

Aug



24 M Biological Logic and Cell Structure
Ch 1 Finish End of Chapter Practice Problems.  These exercise your control of units and simple math.  Remember: a number is meaningless without units. Always remember that answers on exams without units get zero points. Numbers are meaningless without units.   DO THESE PROBLEMS NOW   Solutions Chapter 1 Problems
26 W Water, pKa, Buffers     
Ch 2 End of Chapter Problems here are like that on the Exam...know them NOW.    Solutions Chapter 2 Problems
28 F
Water, pKa, Buffers
Exam 1
Ch  2
Exam 1 during the last 25 minutes of the lecture; 50 points
 31 M
Amino Acids
Ch 3
Problem set exercises not only amino acid chemistry, but sequencing proteins.  Solutions to Chapter 3 Problems
Sept



2 W
Amino Acids, Protein Purification     Ch 3

4 F
Finish Ch3


  7 M
LABOR DAY HOLIDAY


9 W Proteins 3-D Structure Ch4
Problem set here is excellent.  You need to know these well before lectures on Protein Function and Enzymes. 
11 F
Exam 2

Exam 2, 100 points, bring non-graphing calculator.  Integrates material from Chapter 2
  14 M Protein Function Ch 5
 Hemoglobin and Myoglobin are the models for changes of protein strcuture and biological activity/relevance.   Solutions
 16 W
  Enzymes Ch6

Start the problems now, you need to know how to make Michaelis-Menton and Lineweaver-Burk plots:  the exam will have you do these!  Knowing how to graph is simple and necessary!  Problems Solutions
18 F
 Enzymes, con't


21 M
  Enzymes, con't

 
23 W
 Enzymes
Ch7

25 F
 EXAM 3  
100 points.  You need to bring: No 2. Pencils, Simple Scientific Calculator+ Straight Edge (ruler),  This exam integrates material fromChapters 3 and 4.  Exam Key and Distribution After the Exam
Please read course policies about CALCULAOTRS
28 M
Carbohydrates Ch7

30 W
Carbohydrates
Ch 7
Problems with carbohydrate chemistry and glycosidic links:   Get them done NOW!!...and known well before the next exam.
Oct



2 F
Nucleic Acids Ch  8

5 M
Nucleic Adids
Ch 8
Problem set is great with nucleotide and DNA/RNA. 
7 W
Nucleic Acids, con't Ch 8

9 F
Exam 4

100 points, integrates material from Chapter 3 and 6.  Be sure to bring your calculator.
12 M
Lipids Ch 10
Lipids:  fatty acid chemistry and the nature of complex lipids are essential to membrane structure, fats and oil chemistry.  Solutions
14 W
Membranes and Transport Ch 11
Membranes, life would not exist without them...know how to do the problems Now....quick, the exam is coming up.  .
16 F
Membranes and Transpor

19 M
Biosignalling Ch 12
Biosignalling is a membrane based system and phospho-relays.  We will not cover everything in the chapter, just the essentials that relate to the metabolic pathways coming up later.  Know these well, it will help later.  Solutions
 21 W
Biosignalling

 23 F
EXAM 5
Integrates material from Ch 3, 5, 6, 7, 8.  Be sure to bring your calculator.
26 M 
   Bioenergetics   Ch 13
Bioenergetics is simple and striaghtforward, you did all this in General Chem, we just have to make some modifications for Biochem.  All the pathways we will cover next relies upon knowing how enzymes work (Chpt 6) and their bioenergetics.   Problems
 28 W
Bioenergetics, Pathways
Melamine
30 F
Glycolysis, Gluconeogenesis and Pentose Phosphate Pathway Ch 14
es, you have to know perfectly the chemistry of these pathways...practice as often as you can....these are Central Metabolism and all pathways come back to them...they are used throughout the rest of the course.  Solutions
Nov



  2 M
Glyconeogenesis, PPP Ch 14

4 W
 Citric Acid Cycle Ch 16
This is the other Central Metabolism pathway...we will use it extensively in the following chapters.  You need to know it perfectly NOW.   Problem solutions
6 F
Exam 6

100 points.  Be sure to bring your calculator.
9 M
Glucose and Glycogen Regulation
 
Ch 15
All major concepts of pathway regulation are here.  We will be doing them in modified forms for all other pathways.  It is essentail that you can solve the problems now...it makes the rest so much easier!   Solutions

 11 W
VETERANS  DAY  HOLIDAY


 13 F
Fatty acid oxidation   Ch 17
Not Here we go...do the problems and see how Beta-oxidation has parallels to the Citric Acid Cycle besides energizing it!  Problem set is very helpful.    What you need to concentrate on is how nitrogen is dealt with, knowing the Urea cycle perfectly, and generalities of amino acid degradation pathways.     Chapter 17 Solutions     Chapter 18 Solutions
16 M
Fatty acid Oxidation
Ch 17

18 W
Amino acid oxidation Ch18

20 F
 Oxidative Phosphorylation Ch 19
All that NADH and FADH2 ends up here.  Redox chemistry will be practiced (review Chpt 13) and the various poisons of respiration.  Solutions-19
 23 M
  Oxidative Phosphorylation Ch 19

 25 W
Photophosphorylation Ch 19
Electron transport energized by light.  The problem set is great, as well as knowing photosynthetic electron flow and diversity. 
26-29
THANKSGIVING HOLIDY

Gobble, gobble or Oink, oink.
 30 M
Carbohydrate Biosynthesis Ch20
We did glycogen synthesis earlier (Chpt 15), now we get to the other major polysaccharides.  This is easy if you already know carbohydrate structure (Chpt 7) and sugar metabolism (Chpt 14 and 15).   Solutions
  Dec



2,4
Problem Solving


 9
Final EXAM   Note Time:  noon to 2PM.
Comprehensive.  200 points.  After: the graded Exam can be picked up outside OE246.  There will be a key and grade distribution on the bulletin board
PreTestAnswers