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Laboratory Link

General Biochemistry

Fall  -  2008
 
Lectures - MWF  2:00-2:50 PM    VH 131
PreTest
  and  Study Guide   Lecture Links (below) are to Handouts.  Example OLD EXAM.


Instructor Email Phone Office hours
John Makemson
 makemson@fiu.edu
 305-348-3097
 OE 246/7:  MTWTh  11:00 to Noon   and    Friday mornings

Tentative Syllabus

Lecture PowerPoint figures (or Exam Answers and Scores) are available by clicking on the lecture topic for that day. They are all in pdf format.  I





25 M Biological Logic and Cell Structure
Ch 1 Finish End of Chapter Practice Problems.  These exercise your control of units and simple math.  Remember: a number is meaningless without units. Always remember that answers on exams without units get zero points. Numbers are meaningless without units.   DO THESE PROBLEMS NOW   Solutions Chapter 1 Problems
27 W Water, pKa, Buffers     
Ch 2 End of Chapter Problems here are like that on the Exam...know them NOW.    Solutions Chapter 2 Problems
29 F
Water, pKa, Buffers
Exam 1
Ch  2
Exam 1 during the last 25 minutes of the lecture; 50 points
     Sept



       1 M
  Labor Day Holiday


 3 W
Amino Acids     Ch 3
Problem set exercises not only amino acid chemistry, but sequencing proteins.  Solutions to Chapter 3 Problems
5 F
Amino acids, Protein sequencing con't. Ch 3

8 M
Proteins 3-D Structure Ch 4
Problem set here is excellent.  You need to know these well before lectures on Protein Function and Enzymes. 
10 W  Protein Structure+ Review
Ch4

12 F
Exam 2

Exam 2, 100 points, bring non-graphing calculator.  Integrates material from Chapter 2
     15 M
Protein Function Ch 5
 Hemoglobin and Myoglobin are the models for changes of protein strcuture and biological activity/relevance.   Solutions
     17 W
  Enzymes Ch6

Start the problems now, you need to know how to make Michaelis-Menton and Lineweaver-Burk plots:  the exam will have you do these!  Knowing how to graph is simple and necessary!  Problems Solutions
19 F
 Enzymes, con't


22 M
  Enzymes, con't

 
24 W
Carbohydrates Ch7
Problems with carbohydrate chemistry and glycosidic links:   Get them done NOW!!...and known well before the next exam.
26 F
 EXAM 3  
100 points.  You need to bring: No 2. Pencils, Simple Scientific Calculator+ Straight Edge (ruler),  This exam integrates material fromChapters 3 and 4.  Exam Key and Distribution After the Exam
Please read course policies about CALCULAOTRS
29 M
Nucleic Acids Ch 8
Problem set is great with nucleotide and DNA/RNA. 
    Oct



1 W
Nucleic Acids, con't
Ch 8

3 F
Lipids Ch  10
Lipids:  fatty acid chemistry and the nature of complex lipids are essential to membrane structure, fats and oil chemistry.  Solutions
6 M
Membranes and Transport Ch 11
Membranes, life would not exist without them...know how to do the problems Now....quick, the exam is coming up.  .
8 W
Membranes


10 F
Exam 4

100 points, integrates material from Chapter 3 and 6.  Be sure to bring your calculator.
13 M
Biosignalling  Ch 12
Biosignalling is a membrane based system and phospho-relays.  We will not cover everything in the chapter, just the essentials that relate to the metabolic pathways coming up later.  Know these well, it will help later.  Solutions
15 W
Biosignalling
 

17 F
Biosignalling,  Bioenergetics     Ch 12+13
Bioenergetics is simple and striaghtforward, you did all this in General Chem, we just have to make some modifications for Biochem.  All the pathways we will cover next relies upon knowing how enzymes work (Chpt 6) and their bioenergetics.   Problems
20 M
Bioenergetics
  Ch13

     22 W
Bioenergetics, Pathways


      24 F
EXAM 5
Integrates material from Ch 3, 5, 6, 7, 8.  Be sure to bring your calculator.
27 M 
 Glycolysis, Gluconeogenesis and Pentose Phosphate Pathway   Ch 14
Yes, you have to know perfectly the chemistry of these pathways...practice as often as you can....these are Central Metabolism and all pathways come back to them...they are used throughout the rest of the course.  Solutions
 29 W
Glyconeogenesis, PPP


31 F
Citric Acid Cycle   Ch 16
This is the other Central Metabolism pathway...we will use it extensively in the following chapters.  You need to know it perfectly NOW.   Problem solutions
Nov



  3 M
Glucose and Glycogen Regulation Ch 15
All major concepts of pathway regulation are here.  We will be doing them in modified forms for all other pathways.  It is essentail that you can solve the problems now...it makes the rest so much easier!   Solutions
5 W
  Ch 14,15,16
Ch 15-16

7 F
Exam 6

100 points.  Be sure to bring your calculator.
10 M
Amino acid oxidation
 Fatty acid oxidation  
Ch 17+18

Not Here we go...do the problems and see how Beta-oxidation has parallels to the Citric Acid Cycle besides energizing it!  Problem set is very helpful.    What you need to concentrate on is how nitrogen is dealt with, knowing the Urea cycle perfectly, and generalities of amino acid degradation pathways.     Chapter 17 Solutions     Chapter 18 Solutions
 12 W
Fat and AA oxidation


14 F
Oxidative Phosporylation Ch 19
All that NADH and FADH2 ends up here.  Redox chemistry will be practiced (review Chpt 13) and the various poisons of respiration.  Solutions-19
17 M
Photophosphorylation Ch19
Electron transport energized by light.  The problem set is great, as well as knowing photosynthetic electron flow and diversity. 
19 W
 Carbohydrate Biosynthesis Ch 20
We did glycogen synthesis earlier (Chpt 15), now we get to the other major polysaccharides.  This is easy if you already know carbohydrate structure (Chpt 7) and sugar metabolism (Chpt 14 and 15).   Solutions
 21 F
  Ch 20

 24 M



 26 W



27-30
Thanksgiving Holiday, Gobble Gobble or Oink, Oink


  Dec



1,3,5
Problem Solving


  8-11
Final EXAM  Set by Exam Schedule
Comprehensive.  200 points.  After: the graded Exam can be picked up outside OE246.  There will be a key and grade distribution on the bulletin board
PreTestAnswers